Fig. 4.

MALAT1 knockdown inhibited the expression of genes in the AMPK signaling and lipid metabolism pathways through pre-mRNA splicing or transcription.A, the relative RNA levels of MALAT1, SREBF1, SCD, RAB14, PRKAB1, and PRKAG1 were measured by qRT-PCR in HCCLM3 (upper panel) and PLC (lower panel) cell lines. B, the expression of the proteins of interest in HCCLM3 (upper panel) and PLC (lower panel) cells was examined by western blotting. The densities of the corresponding protein bands were measured by Image J and displayed on the right. C, relative quantification of the mRNAs and the corresponding pre-mRNAs by qRT-PCR. D, measurement of the pre-mRNA of SREBF1 by qRT-PCR with or without reverse transcriptase (RT). E, qRT-PCR analysis of the pre-mRNAs copurified with MALAT1 by biotinylated anti-MALAT1 probes. The anti-LacZ probes were used as negative control. Data represent mean ± s.d. of triplicate independent experiments (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, by two-sided Student’s t test).