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. 2021 Sep 8;10:e72286. doi: 10.7554/eLife.72286

Table 1. Yeast strains.

Strain name Protein Genotype
YSD35 CMG MAT a/α ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 pep4::kanMX bar1::hphNAT1 Gal-Gal4 (HIS3) Gal-Mcm5/Mcm4 (TRP1) Gal-Mcm2/FLAG-Mcm3 (URA3) Gal-Mcm7/Mcm6 (LEU2) Gal1-10 Cdc45 (TRP1) Gal1-10 Psf2++/Psf3++ (URA3) Gal1-10 Psf1++/CBP-Sld5++ (LEU2)
YDR154 RNase H2 MATa ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 pep4::kanMX bar::hphNAT1 Gal-Gal4 (HIS3) GAL-CBP-RNH201++ (LEU2) GAL-RNH202++ / RNH203++ (URA3)