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. 2021 Sep 28;3:64. doi: 10.1186/s42523-021-00128-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Oropharyngeal virus shedding from chickens after challenged with H9N2 AIV A/chicken/Pakistan/UDL01/08. a Viral titre recovered from oral swabs at day 2 (D2), 4 (D4), 5 (D5), 6 (D6) post AIV challenge from SPF RIR chickens (Experiment 1). b Viral titre recovered from oral swabs at day 2 (D2), 4 (D4), 5 (D5), 6 (D6) post challenge from SPF RIR and SPF VALO chickens (Experiment 2). Viral titres are expressed as log10 plaque forming units (PFU) per ml. A dashed line indicates the lower limit of detection for the plaque assay carried out on MDCK cells, 0.9 log10 PFU per ml