Table 3.
miRNAs significantly altered by MS and Enrichment
| CPM-Fold Change | p (one-way ANOVA) | ||||||
|---|---|---|---|---|---|---|---|
| Accession ID | Chromosomal Location | miRNA | MS:C | MS + Enr:C | MS + Enr:MS | sig. | |
| upregulated by MS | |||||||
| MIMAT0017819 | chr18: 56726128–56,726,240 | miR-3557-5p | 1.282 | 1.359 | 1.060 | 0.043 | b,d, |
| MIMAT0000838 | chr10: 62014995–62,015,095 | miR-132-3p | 1.278 | 1.394 | 1.091 | 0.000 | a,b,d,f |
| MIMAT0003379 | chr18: 56726150–56,726,214 | miR-378a-3p | 1.276 | 1.362 | 1.068 | 0.019 | a,b,d, |
| MIMAT0026467 | chr11: 16097346–16,097,433 | miR-125b-2-3p | 1.252 | 1.425 | 1.138 | 0.034 | b,d,f |
| MIMAT0017158 | chr10: 62014702–62,014,812 | miR-212-5p | 1.252 | 1.598 | 1.276 | 0.000 | a,b,c,d,f |
| MIMAT0035730 | chr13: 77065446–77,065,507 | miR-1843b-5p | 1.201 | 1.263 | 1.051 | 0.015 | a,b,d, |
| MIMAT0000859 | chr13: 54952903–54,953,012 | miR-181b-5p | 1.195 | 1.373 | 1.149 | 0.004 | a,b,d,f |
| MIMAT0003205 | chr6: 133893419–133,893,495 | miR-409a-3p | 1.176 | 1.324 | 1.125 | 0.000 | a,b,d,f |
| MIMAT0000822 | chr8: 45746948–45,747,027 | miR-100-5p | 1.176 | 1.436 | 1.221 | 0.002 | b,d,f |
| MIMAT0000550 | chr6: 133861199–133,861,284 | miR-323-3p | 1.163 | 1.275 | 1.097 | 0.001 | a,b,d,f |
| MIMAT0000820 | chr11: 16052153–16,052,233 | miR-99a-5p | 1.161 | 1.431 | 1.232 | 0.000 | b,c,d,f |
| MIMAT0017163 | chrX: 3684480–3,684,588 | miR-221-5p | 1.130 | 1.305 | 1.156 | 0.001 | b,c,d,f |
| MIMAT0017217 | chr6: 133699509–133,699,592 | miR-493-5p | 1.124 | 1.220 | 1.085 | 0.046 | b,d,f |
| MIMAT0003202 | chr6: 133884178–133,884,257 | miR-382-3p | 1.123 | 1.310 | 1.167 | 0.002 | b,c,d,f |
| MIMAT0001626 | chr6: 133711425–133,711,538 | miR-431 | 1.118 | 1.225 | 1.096 | 0.002 | a,b,d,f |
| MIMAT0000858 | chr3: 23150352–23,150,468 | miR-181a-5p | 1.117 | 1.343 | 1.203 | 0.021 | b,d,f |
| MIMAT0017123 | chr10: 62014995–62,015,095 | miR-132-5p | 1.115 | 1.249 | 1.120 | 0.002 | a,b,d,f |
| MIMAT0005299 | chr19: 25290051–25,290,133 | miR-181d-5p | 1.113 | 1.399 | 1.257 | 0.000 | b,c,d,f |
| MIMAT0003177 | chr6: 133892655–133,892,744 | miR-541-5p | 1.108 | 1.265 | 1.142 | 0.012 | b,d,f |
| MIMAT0005314 | chr8: 117354821–117,354,903 | miR-425-5p | 1.105 | 1.239 | 1.121 | 0.023 | b,d,f |
| MIMAT0003194 | chr6: 133872439–133,872,522 | miR-376c-3p | 1.103 | 1.185 | 1.074 | 0.002 | a,b,d,f |
| MIMAT0024843 | chr1: 143360224–143,360,287 | miR-1839-5p | 1.103 | 1.237 | 1.122 | 0.014 | b,d,f |
| MIMAT0017870 | chr6: 133893418–133,893,497 | miR-409b | 1.101 | 1.173 | 1.065 | 0.045 | b,d,f |
| MIMAT0003204 | chr6: 133893419–133,893,495 | miR-409a-5p | 1.086 | 1.220 | 1.123 | 0.003 | b,c,d,f |
| MIMAT0004706 | chr1: 59704381–59,704,473 | let-7e-3p | 1.082 | 1.087 | 1.005 | 0.037 | a,b,d, |
| MIMAT0004710 | chr15: 100179879–100,179,962 | miR-17-1-3p | 1.069 | 1.212 | 1.134 | 0.001 | b,c,d,f |
| MIMAT0003200 | chr6: 133877124–133,877,205 | miR-487b-3p | 1.069 | 1.183 | 1.106 | 0.043 | b,d,f |
| MIMAT0003207 | chr6: 133893693–133,893,771 | miR-369-3p | 1.061 | 1.237 | 1.166 | 0.000 | b,c,d,f |
| MIMAT0005282 | chr5: 113657727–113,657,807 | miR-872-5p | 1.046 | 1.193 | 1.141 | 0.001 | b,c,d,f |
| downregulated by MS | |||||||
| MIMAT0000850 | chr10: 65291365–65,291,447 | miR-144-3p | −1.783 | −1.701 | 1.048 | 0.024 | a,d, |
| MIMAT0017154 | chr9: 26791764–26,791,847 | miR-206-5p | −1.766 | −1.707 | 1.035 | 0.008 | a,b,d, |
| MIMAT0005445 | chr4: 58344310–58,344,390 | miR-29b-1-5p | −1.708 | − 1.520 | 1.124 | 0.012 | a,b,d,e, |
| MIMAT0005304 | chr11: 88129426–88,129,503 | miR-301b-3p | −1.564 | − 1.335 | 1.171 | 0.036 | a,d,e, |
| MIMAT0017230 | chr10: 56844977–56,845,045 | miR-497-3p | −1.386 | −1.582 | − 1.142 | 0.001 | a,b,d,f |
| MIMAT0000889 | chr20: 3816158–3,816,267 | miR-219a-5p | −1.314 | −1.504 | − 1.144 | 0.010 | b,d,f |
| MIMAT0000812 | chr7: 123431612–123,431,680 | miR-33-5p | −1.311 | −1.620 | − 1.235 | 0.003 | a,b,d,f |
| MIMAT0001543 | chr2: 44897601–44,897,691 | miR-449a-5p | −1.251 | −1.660 | − 1.327 | 0.001 | b,c,d,f |
| MIMAT0017839 | chr10: 109195234–109,195,336 | miR-3065-5p | −1.238 | −1.139 | 1.087 | 0.045 | a,d, |
| MIMAT0035720 | chr8: 114874457–114,874,544 | let-7 g-3p | −1.231 | −1.288 | − 1.046 | 0.017 | a,b,d, |
| MIMAT0005328 | chr6: 133691381–133,691,463 | miR-673-5p | −1.210 | −1.306 | − 1.080 | 0.000 | a,b,d,f |
| MIMAT0017143 | chr2: 263873759–263,873,844 | miR-186-3p | −1.205 | −1.253 | − 1.040 | 0.015 | a,b,d, |
| MIMAT0017026 | chr10: 74417746–74,417,845 | miR-301a-5p | −1.203 | −1.341 | − 1.115 | 0.008 | a,b,d,f |
| MIMAT0000805 | chr5: 139702872–139,702,963 | miR-30e-5p | −1.178 | −1.238 | − 1.052 | 0.007 | a,b,d,f |
| MIMAT0004707 | chr7: 66802731–66,802,815 | let-7i-3p | −1.168 | −1.326 | − 1.135 | 0.006 | b,d,f |
| MIMAT0000581 | chr10: 109195251–109,195,316 | miR-338-3p | −1.147 | −1.277 | − 1.113 | 0.024 | b,d,f |
| MIMAT0017093 | chr2: 165605923–165,606,020 | miR-15b-3p | −1.144 | −1.174 | − 1.027 | 0.042 | b,d, |
| MIMAT0000587 | chr6: 133733240–133,733,335 | miR-341 | −1.131 | −1.196 | − 1.057 | 0.020 | b,d,f |
| MIMAT0004732 | chr7: 32894877–32,894,976 | miR-135a-3p | −1.114 | −1.189 | − 1.068 | 0.007 | b,d,f |
| MIMAT0000815 | chr5: 167092491–167,092,592 | miR-34a-5p | −1.090 | −1.241 | − 1.139 | 0.036 | b,d,f |
| MIMAT0000570 | chr7: 34881095–34,881,190 | miR-331-3p | −1.067 | −1.315 | − 1.233 | 0.001 | b,c,d,f |
a controls vs. MS
b controls vs. MS + Enr
c MS vs. MS + Enr
d controls vs. MS and MS + Enr
e controls and MS + Enr vs. MS (recovered by Enr)
f controls and MS vs. MS + Enr
Significant changes in miRNA expression after MS and Enr based on one-way ANOVA are shown. Listed miRNAs are split by those upregulated vs. downregulated by MS. CPM-fold change value was calculated as a ratio between two group’s CPM expression. Positive values indicate increased expression and negative values show reduced expression in the numerator group. The full one-way ANOVA significance is listed as p. Specific group differences are listed as sig. Abbreviations: CPM-counts per million, MS-maternal separation, Enr-environmental enrichment, C-control