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. Author manuscript; available in PMC: 2021 Sep 29.
Published in final edited form as: Neuron. 2016 Sep 22;92(1):214–226. doi: 10.1016/j.neuron.2016.09.001

Figure 4. HFS-Induced LTD at D2-VP Synapses Is Presynaptic and DOR Mediated.

Figure 4.

(A) HFS-induced LTD at D2-VP synapses was insensitive to postsynaptic BAPTA (CTRL, 103.54% ± 17.57%, 5 cells from 2 mice; HFS, 61.43% ± 9.92%, 10 cells from 4 mice; HFS with BAPTA, 60.62% ± 6.42%, 6 slices from 3 mice).

(B) HFS-LTD was associated with an increase in the PPR and failure rate and a decrease in C1/V12 (paired t test PPR, t = 2.45, p = 0.035; FR, t = 2.79, p = 0.019; 1/CV2, t = 1.079, p = 0.306, 11 cells from 6 mice).

(C) Twenty individual IPSC traces pre- and post-HFS protocol.

(D) DPDPE induced an LTD in saline-, but not cocaine-treated, mice (SAL, 59.33% ± 7.12%, 10 cells from 3 mice; COC, 97.994% ± 8.14%, 8 cells from 3 mice). Naltrindole (NAL) applied after DPDPE-LTD did not reverse the plasticity (52.92% ± 8.5%, 5 cells from 2 mice).

(E) Incubation in NAL prevented the HFS-induced LTD (CTRL, 61.43% ± 9.92%, 10 cells from 4 mice; NAL, 130.4% ± 8.84%, 9 cells from 5 mice).

(F) DPDPE did not affect transmission at D1-VP synapses (CTRL, 111.85% ± 18.49%, 3 cells from 3 mice; DPDE, 106.99% ± 6.49%, 11 cells from 4 mice); DAMGO (1 μM) induced a depression of IPSCs (61.33% ± 6.08%, 6 cells from 3 mice).

*p < 0.05, all plots mean ± SEM. For representative traces, first and last 20 trials of recording in light and dark orange, respectively. Scale bars, 20 pA, 20 ms. Related to Figure S4.