Fig. 7.

LOX was necessary for the function of miR-30c-2-3p. A, B Effect on cellular migration (A) and invasion (B) by co-transfecting miR-30c-2-3p inhibitor and LOX siRNA into MKN45 and AGS cells. Scale bar = 100 μm. C Tube-formation assays for HUVECs co-cultured with conditioned medium filtrated from treated GC cells. Scale bar = 200 μm. D Western blot analysis of the expression of LOX, EMT markers, VEGFA in AGS cells co-transfected with miR-30c-2-3p inhibitor and LOX-specific siRNA. E, F CCK8 assays for AGS (E) and MKN45 (F) cells as indicated. Data were analyzed using two-way ANOVA. Error bars, mean ± SD from triplicate samples. *P < 0.05, **P < 0.01 by Student’s t‐test unless otherwise specified