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. 2021 Sep 29;129(9):097013. doi: 10.1289/EHP8196

Figure 6.

Figure 6A is a donut chart depicting E R V K equals 29 percent, E R V 1 equals 14 percent, E R V L equals 14 percent, L1 equals 13 percent, E R V L-MaLR equals 7 percent, h A T Charlie equals 5 percent, T c Mar tigger equals 5 percent, h A T tip100 equals 3 percent, and other equals 9 percent. E R V K, E R V 1, E R V L, L1, E R V L-MaL R represent R N A or retroelements, and h A T Charlie, T c Mar tigger, h A T tip100, and other represent D N A transposable element. Figure 6B is a set of five violin plots titled E R V K, E R V 1, E R V L, L1, and E R V L-MaL R plotting read counts (log to the base 10) ranging from 0 to 5 in unit increments (y-axis) for untreated and 100 nanomolar bisphenol A. Figure 6C is a clustered bar graph plotting frequency, ranging from 0 to 60 in increments of 20 (y-axis) across E R V K, E R V 1, L1, Other, E R V L, E R V L-MaL R, h A T Charlie, T c Mar tigger, D N A, h A T tip100 (x-axis) for observed and expected.

Analysis of repeat DNA expression in PGCLCs derived from EpiLCs exposed to 100 nM BPA. (A) Pie chart showing percentage distribution of different repeat families up-regulated in exposed germ cells. For full list of repeat families, see Excel Table S22. (B) Violin plots showing aggregate expression levels of different repeat families indicated, up-regulated in exposed germ cells. Dashed lines represent median and quartiles. Wilcoxon matched-pairs signed rank test p-values: <0.0001 (ERVK, ERV1, ERVL and L1); <0.001 for ERVL-MaR. First plot represents “Untreated” followed by “100 nM exposed.” (C) Bar graph comparing observed with expected frequency of different repeat families indicated, up-regulated in exposed germ cells. Expected frequencies of each family calculated based on total number of upregulated repeat families and proportion occurrence in RepeatMasker database. First plot represents “Observed,” followed by “Expected” frequency. For the full list of up-regulated elements and repeat superfamilies, see Excel Tables S23 and S24, respectively. Note: BPA, bisphenol A; EpiLCs, epiblast-like cell; PGC, primordial germ cell; PGCLC, PGC-like cell.