Table 4.
Ingredients and analyzed dry matter, energy, and nutrients in diets used in experiment 3, as-fed basis1
| Phase 1 diets | Phase 2 diets | Phase 3 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Item, % | No corn protein | No ESBM | No plasma protein | Only corn protein | No corn protein | 5% ESBM | 1% ESBM | Only corn protein | |
| Ingredient, % | |||||||||
| Ground corn | 28.33 | 27.52 | 25.69 | 24.51 | 49.47 | 49.08 | 47.51 | 45.98 | 53.40 |
| Rolled oats | 20.00 | 20.00 | 20.00 | 20.00 | — | — | — | — | — |
| Soybean meal | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 30.00 |
| Corn DDGS | 5.00 | 5.00 | 5.00 | 5.00 | 7.50 | 7.50 | 7.50 | 7.50 | 10.00 |
| Whey, dried | 15.00 | 15.00 | 15.00 | 15.00 | 10.00 | 10.00 | 10.00 | 10.00 | — |
| ESBM | 5.00 | — | 4.50 | — | 7.50 | 5.00 | 1.00 | — | — |
| Plasma protein | 2.50 | 2.50 | — | — | — | — | — | — | — |
| Corn protein | — | 5.00 | 5.00 | 10.00 | — | 2.50 | 7.50 | 10.00 | — |
| Soybean oil | 1.08 | 1.72 | 1.50 | 2.04 | 2.55 | 2.85 | 3.32 | 3.38 | 3.68 |
| Limestone | 1.46 | 1.55 | 1.46 | 1.52 | 1.40 | 1.42 | 1.52 | 1.56 | 1.33 |
| Dicalcium phosphate | 0.28 | 0.20 | 0.30 | 0.25 | 0.20 | 0.18 | 0.10 | 0.05 | 0.30 |
| l-Lys HCl | 0.36 | 0.49 | 0.50 | 0.61 | 0.39 | 0.45 | 0.54 | 0.55 | 0.36 |
| dl-Met | 0.13 | 0.12 | 0.14 | 0.12 | 0.13 | 0.13 | 0.11 | 0.09 | 0.09 |
| l-Thr | 0.12 | 0.14 | 0.15 | 0.17 | 0.12 | 0.14 | 0.14 | 0.13 | 0.10 |
| l-Trp | — | 0.02 | 0.02 | 0.04 | — | 0.01 | 0.02 | 0.02 | — |
| Phytase concentrate2 | 0.02 | 0.02 | 0.02 | 0.02 | 0.02 | 0.02 | 0.02 | 0.02 | 0.02 |
| Toxin binder3 | 0.07 | 0.07 | 0.07 | 0.07 | 0.07 | 0.07 | 0.07 | 0.07 | 0.07 |
| Salt | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 |
| Vitamin–mineral premix4 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 |
| Analyzed dry matter and nutrients, % | |||||||||
| Dry matter | 89.47 | 89.44 | 90.08 | 90.21 | 87.94 | 88.07 | 88.43 | 87.76 | 86.88 |
| Ash | 6.16 | 5.55 | 6.04 | 5.64 | 5.28 | 5.47 | 4.90 | 4.40 | 4.81 |
| Gross energy, kcal/kg | 4,014 | 4,069 | 4,091 | 4,142 | 4,014 | 4,076 | 4,114 | 4,187 | 4,065 |
| Crude protein | 22.76 | 22.46 | 23.79 | 22.39 | 20.27 | 20.92 | 22.20 | 23.13 | 21.15 |
| Acid-hydrolyzed ether extract | 4.36 | 4.95 | 4.91 | 5.67 | 5.65 | 5.86 | 6.98 | 7.86 | 7.82 |
| Ca | 0.89 | 0.86 | 0.96 | 0.80 | 0.68 | 0.74 | 0.69 | 0.69 | 0.75 |
| P | 0.58 | 0.53 | 0.62 | 0.56 | 0.38 | 0.37 | 0.43 | 0.36 | 0.43 |
| Indispensable AA | |||||||||
| Arg | 1.31 | 1.26 | 1.39 | 1.23 | 1.16 | 1.39 | 1.12 | 1.31 | 1.35 |
| His | 0.54 | 0.54 | 0.58 | 0.54 | 0.51 | 0.61 | 0.52 | 0.59 | 0.57 |
| Ile | 0.95 | 0.94 | 1.07 | 0.98 | 0.89 | 1.08 | 0.91 | 0.95 | 0.94 |
| Leu | 1.86 | 1.93 | 2.05 | 2.05 | 1.80 | 2.16 | 1.94 | 2.10 | 1.90 |
| Lys | 1.64 | 1.69 | 1.82 | 1.78 | 1.34 | 1.53 | 1.39 | 1.64 | 1.37 |
| Met | 0.45 | 0.48 | 0.64 | 0.44 | 0.38 | 0.54 | 0.42 | 0.42 | 0.41 |
| Phe | 1.10 | 1.08 | 1.17 | 1.11 | 0.98 | 1.19 | 1.02 | 1.07 | 1.07 |
| Thr | 1.00 | 1.02 | 1.21 | 0.96 | 0.83 | 0.98 | 1.28 | 0.96 | 0.84 |
| Trp | 0.28 | 0.26 | 0.28 | 0.26 | 0.22 | 0.25 | 0.23 | 0.22 | 0.23 |
| Val | 1.14 | 1.15 | 1.19 | 1.12 | 0.98 | 1.19 | 1.02 | 1.11 | 1.06 |
| Dispensable AA | |||||||||
| Ala | 1.05 | 1.11 | 1.18 | 1.19 | 1.04 | 1.23 | 1.13 | 1.25 | 1.11 |
| Asp | 2.12 | 2.04 | 2.33 | 1.98 | 1.85 | 2.29 | 1.85 | 2.00 | 2.07 |
| Cys | 0.41 | 0.44 | 0.46 | 0.38 | 0.32 | 0.43 | 0.35 | 0.36 | 0.34 |
| Glu | 3.80 | 3.79 | 4.12 | 3.91 | 3.47 | 4.22 | 3.58 | 3.87 | 3.77 |
| Gly | 0.87 | 0.87 | 0.94 | 0.88 | 0.79 | 0.93 | 0.80 | 0.90 | 0.89 |
| Ser | 0.98 | 0.99 | 1.05 | 0.99 | 0.85 | 1.02 | 0.89 | 0.97 | 0.93 |
| Tyr | 0.76 | 0.76 | 0.82 | 0.80 | 0.67 | 0.83 | 0.71 | 0.77 | 0.76 |
1DDGS = distillers dried grains with solubles (Flint Hills Resources, Wichita, KS); corn protein (Flint Hills Resources, Wichita, KS); ESBM = enzyme treated soybean meal (Hamlet Protein Inc., Findley, OH); plasma protein (APC Inc., Ankeny, IA).
2Phytase = Quantum Blue 5G (AB Vista, Marlborough, UK). The phytase concentrate contained 5,000 phytase units per gram. At 0.02% inclusion, the concentrate provided 1,000 units of phytase per kilogram of the complete diet.
3Toxin binder, hydrated sodium calcium aluminosilicate (United Animal Health, Sheridan, IN).
4Provided the following quantities of vitamins and micro-minerals per kilogram of complete diet: Vitamin A as retinyl acetate, 11,136 IU; vitamin D3 as cholecalciferol, 2,208 IU; vitamin E as dl-alpha tocopheryl acetate, 66 IU; vitamin K as menadione dimethylprimidinol bisulfite, 1.42 mg; thiamin as thiamine mononitrate, 0.24 mg; riboflavin, 6.59 mg; pyridoxine as pyridoxine hydrochloride,0.24 mg; vitamin B12, 0.03 mg; d-pantothenic acid as d-calcium pantothenate, 23.5 mg; niacin, 44.1 mg; folic acid, 1.59 mg; biotin, 0.44 mg; Cu, 20 mg as copper chloride; Fe, 126 mg as ferrous sulfate; I, 1.26 mg as ethylenediamine dihydroiodide; Mn, 60.2 mg as manganese hydroxychloride; Se, 0.3 mg as sodium selenite and selenium yeast; and Zn, 125.1 mg as zinc hydroxychloride.