FIG. 1.

Sequences of his4-C48 and his4-C12 insertions and position of these insertions relative to the HIS4 coding sequence. Although the his4-C48 insertion has only 47 copies of CCGNN, we use the his4-C48 term to keep the nomenclature consistent with the previous study (46). The HIS4 and BIK1 genes are boxed, with the direction of transcription indicated by arrows. The oval shows the position of the HIS4 TATA sequence. The CCGNN repeats replace the upstream regulatory sequences of HIS4 and a portion of the neighboring BIK1 gene as shown. The polylinker sequences are underlined. Sequences: a, (CCGNN)₄₈ insertion (his4-C48); b, (CCGNN)₁₂ insertion (his4-C12).