Table 1.
Kinetic properties of P5C synthetase 2 from rice.
| Vmax (Glu) | 17.38±0.52 | nkat (mg protein)−1 |
| Vmax (ATP) | 17.96±0.46 | nkat (mg protein)−1 |
| Vmax (NADPH) steady-state rate | 18.36±0.64 | nkat (mg protein)−1 |
| KM(app) for L-Glu | 2.69±0.19 | mM |
| KM(app) for ATP* | 0.764±0.067 | mM |
| KM(app) for NADPH steady-state rate | 0.045±0.004 | mM |
*
Value calculated from the Lineweaver-Burk plot of results obtained by considering ATP concentrations≤4mM. When all the results shown inFigure 3Bwere considered using the inhibition model, a value of 1.29±0.20 was instead found.