Fig. 4. Binding profiles for tri-, tetra- and penta-ADPr to the recombinant, purified human ALC1 macrodomain module. (A) Tycho-thermal shift analysis of unliganded ALC1 macrodomain module (green line), as well as in the presence of tri-ADPr (blue line), tetra-ADPr (red line) and penta-ADPr (black line). (B) ITC assays for tri-ADPr (blue triangles), tetra-ADPr (red circles) and penta-ADPr (black squares). (C) Thermodynamic fitting parameters for the binding of tri-, tetra- and penta-ADPr to the ALC1 macrodomain module as determined by ITC.
