REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rat anti-PylRS	(Nikić et al., 2016)	RRID: AB_2893022
Mouse anti-HA	Sigma-Aldrich	Cat# H9658; RRID: AB_260092
Rabbit anti-Myc	Abcam	Cat# ab9106; RRID: AB_307014
Rabbit anti-AIF	Abcam	Cat# ab32516; RRID: AB_726995
Rabbit anti-Giantin	Abcam	Cat# ab80864; RRID: AB_10670397
Rabbit anti-Calnexin	Abcam	Cat# ab22595, RRID: AB_2069006
Rabbit anti-NaK-ATPase	Abcam	Cat# ab76020; RRID: AB_1310695
Rabbit anti-RPL26L1	Abcam	Cat# ab137046; RRID: AB_2893020
Goat anti-rat-Alexa Fluor 405	Abcam	Cat# ab175671; RRID: AB_2890626
Goat anti-rat-Alexa Fluor 405	Abcam	Cat# ab175673; RRID: AB_2893021
Goat anti-rabbit-Alexa Fluor 647	ThermoFisher	Cat# A-21246; RRID: AB_2535814
Goat anti-mouse-Alexa Fluor 647	ThermoFisher	Cat# A32728; RRID: AB_2633277
Goat anti-mouse-Alexa Fluor 405	ThermoFisher	Cat# A-31553; RRID: AB_221604
Chemicals, peptides, and recombinant proteins
Cyclooctyne lysine (SCO-K)	SiChem	SC-8000
3-iodophenylalanine (3-IF)	Chem-Impex International Inc.	14352
Cyclooctyne tyrosine (SCO-Y)	This paper	N/A
Axial trans-cyclooct-2-ene-lysine (TCO∗A-K)	SiChem	SC-8004
N-ε-benzyloxycarbonyl-lysine (Cbz-K)	Sigma-Aldrich	96840
DAPI	Sigma-Aldrich	D9542
Hoechst 33342	Sigma-Aldrich	B2261
Catalase	Sigma-Aldrich	C3155
Glucose oxidase	Sigma-Aldrich	G7141
β-Mercaptoethylamine	Sigma-Aldrich	411000
Polyethyleneimine	Sigma-Aldrich	408727
H-Tet-SiR (silicone rhodamine tetrazine)	Spirochrome	SC008
VRC (ribonucleoside vanadyl complexes)	Sigma-Aldrich	94740
E. coli tRNA	Sigma-Aldrich	R1753
Dextran sulfate	Sigma-Aldrich	D8906
Experimental models: cell lines
HEK293T cells	ATCC	CRL-3216
Oligonucleotides
5′-(Cy5)-ATCTTGAAGTTGGCCTTGATGCCGTT CTTCTGCTT-3′	IBA GmbH	N/A
5′-(Alexa647)-CTGCAGACATGGGTGATCCTCA TGTTTTCTA-3′	IBA GmbH	N/A
5′-(Alexa647)-CTAACCCGGCTGAACGGATTTA GAGTCCATTCGATC-3′	Integrated DNA Technologies	N/A
Recombinant DNA
Plasmids described in Constructs, cloning and mutagenesis, see Table S1 for further details	This paper	N/A
UbC NLS-HA-MCP-YFP	Grünwald and Singer, 2010	Addgene #31230
pDEST_FUS	Hoell et al., 2011	Addgene #26374
pDEST_EWSR1	Hoell et al., 2011	Addgene #26377
pcDNA3.1/Zeo	ThermoFisher	V86020
pBI-CMV1	Clontech	631630
Software and algorithms
FlowJo	BD	https://www.flowjo.com
Fiji	Schindelin et al., 2012	https://fiji.sc/
arivis Vision4D	arivis AG	https://imaging.arivis.com/en/imaging-science/arivis-vision4d
Igor Pro	WaveMetrics	https://www.wavemetrics.com/
Prism	GraphPad	https://www.graphpad.com/
ChemDraw 20.0	PerkinElmer	https://perkinelmerinformatics.com/products/research/chemdraw/
IsobarQuant	Franken et al., 2015	https://codeload.github.com/protcode/isob/zip/1.0.0
Mascot V2.4	Matrix Science	http://www.matrixscience.com/mascot_support_v2_4.html
Adobe Illustrator 2021, Adobe Premiere Pro 2020	Adobe	https://www.adobe.com/creativecloud.html
Other
RFP-Trap Magnetic Agarose	ChromoTek	rtma-20
GFP-Trap Magnetic Agarose	ChromoTek	gtma-20