Figure 1.

Cell-intrinsic role of IL-27 in promoting CD8αα⁺CD4⁺ IEL differentiation. (A) Frequencies of small intestinal CD8αα⁺CD4⁺ IELs in naive WT mice over time. (B–D) Representative FACS profiles with gating strategy for different IEL subsets (B) and frequencies of CD8α⁺ in TCRβ⁺TCRγδ⁻CD4⁺ cells (C) and TCRβ⁺TCRγδ⁻CD8α⁺CD4⁺ cells (D) in total IELs from young (∼10–12 wk) T-rKO mice and WT littermates. (E–J) Representative FACS profiles with gating strategy for different IEL subsets (E) and frequencies of CD8α⁺ in TCRβ⁺TCRγδ⁻CD4⁺ cells (F) and TCRβ⁺TCRγδ⁻CD8α⁺CD4⁺ in total IELs (G), as well as frequencies of TCRαβ⁺CD8αβ⁺(H), TCRαβ⁺CD8αα⁺ (I), and TCRγδ⁺CD8αα⁺ (J) IEL subsets from old (>6 mo) T-rKO mice and WT littermates. Each symbol represents an individual mouse, and the bar represents the mean. Data are pooled from at least three independent experiments. Results of Student’s t test: ***, P < 0.001.