Figure 2.

IL-27 produced by either cDCs or other myeloid cells does not contribute to the differentiation of the CD8αα⁺CD4⁺ IEL subset but promotes T-bet⁺ T reg cells and IL-10 production, respectively. (A) FACS analysis and frequencies of T-bet⁺ T reg cells in LP of T reg-rKO mice and WT littermates (∼10–12 wk). (B–F) Frequencies of CD8α⁺ in TCRβ⁺TCRγδ⁻CD4⁺ cells (B) and TCRβ⁺TCRγδ⁻CD8α⁺CD4⁺ (C) in total IELs as well as frequencies of TCRαβ⁺CD8αβ⁺ (D), TCRαβ⁺CD8αα⁺ (E), and TCRγδ⁺CD8αα⁺ (F) IEL subsets from old (>6 mo) DC-KO mice and WT littermates. (G) FACS analysis and frequencies of T-bet⁺ T reg cells in LP of Mye-KO mice and WT littermates (∼10–12 wk). (H–N) FACS analysis and frequencies of IL-10⁺ conventional T cells in LP of Mye-KO mice (H), DC-KO mice (I), and their corresponding WT littermates (∼10–12 wk). Frequencies of CD8α⁺ in TCRβ⁺TCRγδ⁻CD4⁺ cells (J) and TCRβ⁺TCRγδ⁻CD8α⁺CD4⁺ in total IELs (K), as well as frequencies of TCRαβ⁺CD8αβ⁺ (L), TCRαβ⁺CD8αα⁺ (M), and TCRγδ⁺CD8αα⁺ (N) IEL subsets from old (>6 mo) Mye-KO mice and WT littermates. Each symbol represents an individual mouse, and the bar represents the mean. Data are pooled from at least three independent experiments. Results of Student’s t test: *, P < 0.05; **, P < 0.01.