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. 2021 Sep 23;218(11):e20210021. doi: 10.1084/jem.20210021

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© 2021 Lin et al.

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Figure 5. — Defective CD8αα⁺CD4⁺ IEL responses in IEC-KO are responsible for impaired parasite control during T. gondii infection. (A) FACS analysis and frequencies of small intestinal CD8αα⁺CD4⁺ IELs in IEC-KO mice and WT littermates (>6 mo) with or without adoptive transfer of exogenous CD8αα⁺CD4⁺ IELs. (B) FACS analysis and frequencies of total CD8αα⁺CD4⁺ IELs in IEC-KO mice (>6 mo) at different time points after adoptive transfer of exogenous CD8αα⁺CD4⁺ IELs. (C) FACS analysis and frequencies of CD45.1⁺CD8αα⁺CD4⁺ IELs in IEC-KO mice (>6 mo) at different time points after adoptive transfer of exogenous CD8αα⁺CD4⁺ IELs. (D) qPCR analysis of parasite burden in small intestine (SI) and liver of IEC-KO and WT littermates with or without adoptive transfer of exogenous CD8αα⁺CD4⁺ IELs at day 8 after T. gondii infection. (E–I) qPCR analyses for the expressions of Il27ra (E), Ido1 (F), and Dmbt1 (G) in small intestinal CD45⁻EpCAM⁺ IECs and qPCR analysis of parasite burden in small intestine (H) and liver (I) of 8–12-wk-old IEC-rKO and WT littermates at day 8 after T. gondii infection. Each symbol represents an individual mouse, and the bar represents the mean. Data are pooled from at least three independent experiments. Results of one-way ANOVA in A–D and Student’s t test in E–I: ***, P < 0.001.