Figure S1.

IEC-KO mice do not exhibit a CD8αα⁺CD4⁺ IEL phenotype at a young age. (A–C) Representative FACS profiles with gating strategy (A) for different IEL subsets and frequencies of CD8α⁺ in TCRβ⁺TCRγδ⁻CD4⁺ cells (B) and (C) TCRβ⁺TCRγδ⁻CD8α⁺CD4⁺ in total IELs (C) from young (∼10–12 wk) IEC-KO mice and WT littermates. Each symbol represents an individual mouse, and the bar represents the mean. Data are pooled from at least three independent experiments. Results of Student’s t test: n.s., not significant.