FIG. 5.

Radiation sensitivity of synchronous mre11-3 cultures. Methods of synchronization and irradiation are as described in the legend to Fig. 3 and Materials and Methods. IR dose = 150 Gy. Haploid mre11Δ strain JPY69 was transformed with an ADH1 promoter-driven MRE11 or mre11-3 expression construct (DB-MRE11-TRP or DB-mre11-3-TRP) or an empty vector (DB-P-TRP) (7). Asynchronous, G₁-synchronous, and G₂-synchronous cultures of JPY69 transformants were plated onto SC-Trp media and scored for cell survival for 5 days following irradiation. Values plotted represent the averages of triplicate platings. Error bars represent standard deviations. The standard deviation for the asynchronous MRE11 culture (1.2%) is not visible at the scale of this plot.