TABLE 3.
Radiation-induced interhomologue recombination in mre11Δ/mre11Δ transformants
| Plasmida | IR-induced frequency (n)b
|
|||||
|---|---|---|---|---|---|---|
| 50 Gy
|
150 Gy
|
|||||
| Asynchronous | G1 arrested | G2 arrested | Asynchronous | G1 arrested | G2 arrested | |
| pScM11-314 | 7.7 ± 2.5 (14) | 33.5 ± 16.7 (16) | 7.1 ± 3.5 (16) | 7.3 ± 1.4 (5) | 35.0 ± 8.4 (6) | 4.8 ± 2.2 (6) |
| pRS314 | 11.1 ± 12.1 (10) | 49.0 ± 15.6 (8) | 8.5 ± 15.9 (8) | −4.7 ± 1.9 (6) | 31.5 ± 6.9 (5) | −3.2 ± 6.9 (6) |
a
Diploid mre11Δ strain JPY264 was transformed with a native promoter-driven MRE11 expression construct (pScM11-314) or an empty vector (pRS314). Details of plasmid construction are given in Materials and Methods.
b
IR-induced frequencies were calculated by subtracting the spontaneous frequency of canavanine-resistant methionine prototrophs in the unirradiated sample from the frequency in the irradiated sample. Values are given as 10−4 recombinants per viable cell ± standard deviations. Numbers in parentheses indicate the number of independent cultures used to determine the given value.