Fig 6. Similar relative frequencies (RFs) of tprK variable region alleles are observed across culture replicates.

(A) Comparison of relative frequencies of alleles between two sets of culture passages. Dots represent unique variable region sequences of varying timepoints, color-coded by region. Dotted gray line plots Y = X. (B) Comparison of relative frequencies of all novel alleles not in inoculum between two culture replicates, color-coded by variable region. (C) Relative frequencies of alleles between both culture replicates, at over 70%. Data are grouped by variable region and color coded by week. (D) Correlation between relative frequencies of alleles in culture replicates at low frequencies over time. Dots represent unique variable region sequences between timepoints, with corresponding regression lines plotted. Both are color-coded by week.