Table 2. Primers and probes used for qPCR, Standard PCR and sequencing in this study.
| Microorganisms | Targeted gene | Primers F, R (5’-3’) and Probes S (6FAM–TAMRA) | Tm | References |
|---|---|---|---|---|
| Piroplasmida | 5.8S rRNA 18S rRNA |
5.8S-F5-AYYKTYAGCGRTGGATGTC 5.8S-R-TCGCAGRAGTCTKCAAGTC 5.8S-S-TTYGCTGCGTCCTTCATCGTTGT piro18SF1- GCGAATGGCTCATTAIAACA piro18SF4-TTTCAGMCTTGCGACCATACT piro18SF3-GTAGGGTATTGGCCTACCG piro18SR3-AGGACTACGACGGTATCTGA |
- 58°C |
[135] |
| Anaplasmataceae | 23S rRNA (TtAna) 23S rRNA Ana-rpoB |
TtAna-F-TGACAGCGTACCTTTTGCAT TtAna-R-GTAACAGGTTCGGTCCTCCA TtAna-S-CTTGGTTTCGGGTCTAATCC Ana23S-212F-GTTGAAAARACTGATGGTATGCA Ana23S-753R-TGCAAAAGGTACGCTGTCAC rpoB-F-GCTGTTCCTAGGCTYTCTTCGCGA rpoB-R-AATCRAGCCAVGAGCCCCTRTAWGG |
- 55°C 52°C |
[24] |
| Rickettsia sp. |
gltA (RKNDO3) gltA OmpB |
RKNDO3-F-GTGAATGAAAGATTACACTATTTAT RKNDO3-R-GTATCTTAGCAATCATTCTAATAGC RKNDO3-S-CTATTATGCTTGCGGCTGTCGGTTC CS2D-ATGACCAATGAAAATAATAAT CSEnd-CTTATACTCTCTATGTACA 120-M59-CCGCAGGGTTGGTAACTGC 120-607-AATATCGGTGACGGTCAAGG 120-1497- CCTATATCGCCGGTAATT |
- 50°C 50°C |
[136]
[137] [138] |
| Borrelia sp. | Internal transcribed spacer 16S RNA (Bor ITS4) 16S rRNA |
BorITS4-F-GGCTTCGGGTCTACCACATCTA BorITS4-R-CCGGGAGGGGAGTGAAATAG BorITS4-S-TGCAAAAGGCACGCCATCACC 16S-F-GCTGGCAGTGCGTCTTAAGC 16S-R-GCTTCGGGTATCCTCAACTC |
- 57°C |
[139] |
| Coxiella burnetii | Insertion Sequence (IS1111) Cox2 Cox5 Cox18 |
IS1111-F-CAAGAAACGTATCGCTGTGGC IS1111-R-CACAGAGCCACCGTATGAATC IS1111-S-CCGAGTTCGAAACAATGAGGGCTG Cox2-F-CAACCCTGAATACCCAAGGA Cox2-R-GAAGCTTCTGATATAGGCGGGA Cox5-F-CAGGAGCAAGCTTGAATGCG Cox5-R-TGGTATGACAACCCGTCATG Cox18-F-CGCAGACGAATTAGCCAATC Cox18-R-TTCGATGATCCGATGGCCTT |
- 57°C |
[63]
[140] |
| Bartonella sp. | Internal transcribed spacer16S (BartoITS3) | BartoIRS3-F-GATGCCGGGGAAGGTTTTC BartoIRS3-R-GCCTGGGAGGACTTGAACCT BartoIRS3-S-GCGCGCGCTTGATAAGCGTG |
- | [141] |
| Filariidae | Pan-fil 28S rRNA Triplex TaqMan Cox1 SSU rRNA (18S) |
qFil-28S-F-TTGTTTGAGATTGCAGCCCA qFil-28S-R-GTTTCCATCTCAGCGGTTTC qFil-28S-S-CAAGTACCGTGAGGGAAAGT Fil.COI.749-F-CATCCTGAGGTTTATGTTATTATTTT D.imm.COI.777-S-CGGTGTTTGGGATTGTTAGTG D.rep.COI.871-S-TGCTGTTTTAGGTACTTCTGTTTGAG Fwd.18S.631-TCGTCATTGCTGCGGTTAAA Rwd.1465-GGTTCAAGCCACTGCGATTAA |
- 55°C |
[142]
[143] [144] |