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. 2021 Sep 29;7(40):eabj3658. doi: 10.1126/sciadv.abj3658

Fig. 3. Endogenous L1 is required for Alu cDNA synthesis.

Fig. 3.

(A) Endogenous L1 mRNA abundance, monitored by real-time polymerase chain reaction (PCR) in the macular RPE of human GA eyes (n = 8) compared with normal human eyes (n = 5). *P < 0.05 by Mann-Whitney U test. Error bars show SEM. (B) Immunoblotting analysis of cell extracts from macular RPE from individual human donor eyes. Lysates were used to detect endogenous L1 ORF2p. (C) Fundus photographs and RPE morphology stained for zonula occludens-1 (ZO-1; red) in R. norvegicus (WT rat) or O. palustris (Oryzomys) following Alu RNA administration. n = 5 to 9. (D) Fundus photographs and RPE morphology stained for ZO-1 in O. palustris after Alu RNA administration following enforced expression of rat L1 ORF2p or rat L1 ORF1p. n = 5 to 9. Binary and morphometric quantification of RPE degeneration are shown (*P < 0.05; ***P < 0.001). PM, polymegethism [mean (SEM)]. Arrowheads in fundus images denote the boundaries of RPE hypopigmentation.