Table 2. Primary antibodies used for IHC, with staining performed on the Leica BOND RX Autostainer.
Antibody | Company | Product | Dilution | Incubation (min) |
Pan-cytokeratin (C-11) | NeoMarkers | 149-P | 1:200 | 60 |
Ki67 (SP6) | Thermo Fisher Scientific | RM-9106-S1 | 1:500 | 60 |
CC3 | Cell Signaling Technology | 9661 | 1:200 | 60 |
pTyr397-FAK | Abcam | ab39967 | 1:4000 | 30 |
Merlin (NF2) (D1D8) | Cell Signaling Technology | 6995 | 1:1000 | 60 |
GFP | Invitrogen | A11122 | 1:1000 | 60 |