Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | C57BL/6J mice | https://www.jax.org/strain/000664 | RRID:IMSR_JAX:000664 | |
| Cell line (Homo sapiens) | HEK-293T | ATCC | ATCC:CRL-3216; RRID:CVCL_0063 | |
| Other | Rat-tail Collagen I | Corning | Corning:354236 | |
| Other | Growth factor reduced Matrigel | Corning | Corning:356231 | |
| Other | DMEM/F12 | Sigma | Sigma:D6434 | |
| Other | L-Glutamine | Sigma | Sigma:G7513 | |
| Other | Penicillin/Streptomycin | Sigma | Sigma:P4333 | |
| Other | DNAse I | Roche | Roche:10104159001 | |
| Other | 10% BSA | Sigma | Sigma:A1595 | |
| Other | MEM Non-Essential Amino Acids solution | Gibco | Thermo Fisher Scientific:11140050 | |
| Other | Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) | Gibco | Thermo Fisher Scientific:51500056 | |
| Other | Primocin | Invivogen | Invivogen:ant-pm-1 | |
| Other | 10x DMEM | Sigma | Sigma:D2429 | |
| Chemical compound, drug | 1 N NaOH | Sigma | Sigma:S2770 | |
| Peptide, recombinant protein | Human recombinant FGF10 | R&D Systems | R&D Systems:345-FG | |
| Peptide, recombinant protein | Human recombinant FGF7 | Peprotech | Peprotech:100–19 | |
| Other | CryoStor CS10 | Stem Cell Technologies |
Stem Cell Technologies:07959 |
|
| Other | Normal donkey serum | Jackson ImmunoResearch | Jackson Immuno Research:017-000-121; RRID:AB_2337258 |
|
| Antibody | Anti-SOX9 (rabbit polyclonal) | Millipore | Millipore:AB5535; RRID:AB_2239761 | (1:250) |
| Antibody | Anti-KI67 (rat monoclonal) | Invitrogen | Thermo Fisher Scientific:14-5698-82; RRID:AB_10854564 |
(1:250) |
| Antibody | Anti-SOX2 (goat polyclonal) | R&D Systems | R&D Systems:AF2018; RRID:AB_355110 | (1:250) |
| Antibody | Anti-KRT5 (rabbit polyclonal) | Abcam | Abcam:ab53121; RRID:AB_869889 | (1:250) |
| Antibody | Anti-FOXJ1 (mouse monoclonal) | Invitrogen | Thermo Fisher Scientific:14-9965-82; RRID:AB_1548835 |
(1:250) |
| Antibody | Anti-ProSP-C (rabbit polyclonal) | Millipore | Millipore:AB3786; RRID:AB_91588 | (1:500) |
| Antibody | Anti-RAGE (rat monoclonal) | R&D Systems | R&D Systems:MAB1179; RRID:AB_2289349 | (1:250) |
| Antibody | Anti-PDPN (sheep polyclonal) | R&D Systems | R&D Systems:AF3670; RRID:AB_2162070 | (1:250) |
| Antibody | Anti-PDPN (syrian hamster monoclonal) | DSHB | DSHB:8.1.1; RRID:AB_531893 |
(1:20) |
| Antibody | Anti-PODXL (goat polyclonal) | R&D System | R&D Systems:AF1556; RRID:AB_354858 | (1:250) |
| Antibody | Anti-ZO1/TJP1 (mouse monoclonal) |
Invitrogen | Thermo Fisher Scientific:33–9100; RRID:AB_2533147 |
(1:250) |
| Antibody | Anti-CDH1 (goat polyclonal) | R&D Systems | R&D Systems:AF748; RRID:AB_355568 | (1:250) |
| Antibody | Anti-LAMP3 (rat monoclonal) | Dendritics | IMGENEX:DDX0192; RRID:AB_1148779 | (1:250) |
| other | Phalloidin, Alexa Fluor 488 conjugate |
InvitrogenThermo Fisher Scientific:A12379 | Thermo Fisher Scientific:A12379 | (1:500) |
| other | DAPI | Sigma | Sigma:D9542 | (1 µg/ml) |
| Antibody | Alexa 488-, 568-, 647- or Cy3-conjugated secondaries |
Invitrogen | (1:500) | |
| Antibody | Alexa 488-, 568-, 647- or Cy3-conjugated secondaries |
Jackson ImmunoResearch |
(1:500) | |
| Other | DMEM + Glutamax | Gibco | Thermo Fisher Scientific:31966021 | |
| Other | FBS superior | Sigma | Sigma:S0615 | |
| Other | Lipofectamine 3000 Transfection Reagent | Invitrogen | Thermo Fisher Scientific:L3000001 | |
| Transfected construct (Homo sapiens) | M50 Super 8x TOPFlash (plasmid) | Addgene | Addgene:12456; http://n2t.net/addgene:12456; RRID:Addgene_12456 | |
| Transfected construct (Homo sapiens) | M51 Super 8x FOPFlash (TOPFlash mutant) (plasmid) | Addgene | Addgene:12457; http://n2t.net/addgene:12457; RRID:Addgene_12457 | |
| Transfected construct (Homo sapiens) | pRL-TK (plasmid) | Promega | Promega:E2241 | |
| Peptide, recombinant protein | Recombinant human WNT3A | Proteintech | Proteintech:HZ-1296 | (500 ng/ml) |
| Chemical compound, drug | Dimethyl sulfoxide (DMSO) | Sigma | Sigma:D2650 | |
| Chemical compound, drug | Emodin | Tocris | Tocris:3811 | (10 µM) |
| Chemical compound, drug | TTP22 | Tocris | Tocris:4432 | (10 µM) |
| Chemical compound, drug | IC261 | Sigma | Sigma:I0658 | (10 µM) |
| Chemical compound, drug | CX-4945 | Enzo Life Sciences | Enzo Life Sciences: ENZ-CHM151 |
(10 µM) |
| Chemical compound, drug | DMAT | Sigma | Sigma:SML2044 | (10 µM) |
| Chemical compound, drug | IWR-1 | Sigma | Sigma:I0161 | (10 µM) |
| Commercial assay or kit | Dual-Luciferase Reporter Assay System |
Promega | Promega:E1910 | |
| Commercial assay or kit |
NucleoSpin RNA kit | Macherey-Nagel | Macherey- Nagel:740955.50 |
|
| Commercial assay or kit |
Superscript III Reverse Transcriptase system |
Invitrogen | Thermo Fisher Scientific:18080093 | |
| Commercial assay or kit |
DyNAmo ColorFlash SYBR green qPCR kit | Thermo Scientific | Thermo Fisher Scientific:F416XL | |
| Sequence- based reagent |
qPCR | This paper | Supplementary file 1 | |
| Software, algorithm |
Fiji/ImageJ 1.53 c | Schindelin et al., 2012; doi:10.1038/nmeth.2019 | RRID:SCR_002285 | |
| Software, algorithm |
GraphPad Prism 8 | GraphPad | RRID:SCR_002798 |