| Transfected construct (human) |
ACE2 WT |
Oxford Protein Production Facility-UK |
pOPINTTGneo_ACE2-BAP |
T |
| Transfected construct (human) |
ACE2 S19P; ACE2 K26R |
This paper |
|
Available from authors |
| Transfected construct (SARS-CoV-2) |
RBD WT |
BEI Resources, NIH |
NR-52309 |
pCAGG plasmid |
| Transfected construct (SARS-CoV-2) |
RBD K417N; RBD RBD K417T; RBD S477N; RBD E484K; RBD N501Y; RBD K417N/E484K; RBD K417T/E484K; RBD beta; RBD gamma |
This paper |
|
pCAGG plasmid. Available from authors |
| Transfected construct (human) |
pTT3-BirA-FLAG |
Addgene |
RRID:Addgene_64395
|
Cotranfected for in-cell biotinylation |
| Peptide, recombinant protein |
ACE2 WT; ACE2 S19P; ACE2 K26R |
This paper |
|
Expressed in HEK293 cells and purified |
| Peptide, recombinant protein |
RBD WT; RBD K417N; RBD K417T; RBD S477N; RBD E484K; RBD N501Y; RBD K417N/E484K; RBD K417T/E484K; RBD beta; RBD gamma |
This paper |
|
Expressed in HEK293 cells and purified |
| Antibody |
anti-human ACE2 (mouse monoclonal) |
NOVUS Biologicals |
AC384 |
(5 µg/mL) |
| Cell line (human) |
FreeStyle HEK293F Cells |
ThermoFisher Scientific |
RRID:CVCL_D603
|
|
| Chemical compound, drug |
FreeStyle MAX Reagent |
ThermoFisher |
16447100 |
|
| Chemical compound, drug |
FreeStyle 293 Expression Medium |
ThermoFisher |
12338018 |
|
| commercial assay or kit |
QuikChange II XL |
Agilent |
200,521 |
|
| Commercial assay or kit |
Amine coupling kit |
Cytiva |
BR100050 |
|
| Software, algorithm |
GraphPad |
Prism |
Version 9 |
|
| Other |
CM5 sensor chips |
Cytiva |
29149603 |
|
