| Cell line (Homo sapiens) |
HEK 293T |
ATCC |
ATCC Cat# CRL-3216, RRID:CVCL_0063
|
|
| Cell line (Homo sapiens) |
A549 |
ATCC |
ATCC Cat# CCL-185, RRID:CVCL_0023
|
|
| Cell line (Melanochromis auratus) |
BHK-21 |
ATCC |
ATCC Cat# CCL-10, RRID:CVCL_1915
|
|
| Strain, strain background (Escherichia coli) |
SW102 |
NCI Preclinical Repository |
|
Used for BAC recombineering |
| Recombinant DNA reagent |
RSV BAC |
BEI Resources |
NR-36460 |
This BAC has been further modified for this work as described in Materials and methods |
| Recombinant DNA reagent |
pA2-Lopt |
BEI Resources |
NR-36461 |
|
| Recombinant DNA reagent |
pA2-Nopt |
BEI Resources |
NR-36462 |
|
| Recombinant DNA reagent |
pA2-Popt |
BEI Resources |
NR-36463 |
|
| Recombinant DNA reagent |
pA2-M2-1opt |
BEI Resources |
NR-36464 |
|
| Recombinant DNA reagent |
pCAGGS-T7 RNAP |
This work |
|
Plasmid encoding T7 RNAP |
| Recombinant DNA reagent |
pmAb-ADI-14353 HC |
This work |
|
Plasmid for expressing recombinant antibody |
| Recombinant DNA reagent |
pmAb-ADI-14353 LC |
This work |
|
Plasmid for expressing recombinant antibody |
| Recombinant DNA reagent |
pmAb-ADI-14359 HC |
This work |
|
Plasmid for expressing recombinant antibody |
| Recombinant DNA reagent |
pmAb-ADI-14359 HC Fab |
This work |
|
Plasmid for expressing recombinant antibody |
| Recombinant DNA reagent |
pmAb-ADI-14359 LC |
This work |
|
Plasmid for expressing recombinant antibody |
| Recombinant DNA reagent |
pmAb-ADI-19425 HC |
This work |
|
Plasmid for expressing recombinant antibody |
| Recombinant DNA reagent |
pmAb-ADI-19425 LC |
This work |
|
Plasmid for expressing recombinant antibody |
| Recombinant DNA reagent |
pmAb-5C4 HC (IgG1) |
This work |
|
Plasmid for expressing recombinant antibody |
| Recombinant DNA reagent |
pmAb-5C4 HC (IgM) |
This work |
|
Plasmid for expressing recombinant antibody |
| Recombinant DNA reagent |
pmAb-5C4 LC |
This work |
|
Plasmid for expressing recombinant antibody |
| Recombinant DNA reagent |
pmAb-CR9501 HC |
This work |
|
Plasmid for expressing recombinant antibody |
| Recombinant DNA reagent |
pmAb-CR9501 LC |
This work |
|
Plasmid for expressing recombinant antibody |
| Recombinant DNA reagent |
pmAb-Motavizumab HC |
This work |
|
Plasmid for expressing recombinant antibody |
| Recombinant DNA reagent |
pmAb-Motavizumab LC |
This work |
|
Plasmid for expressing recombinant antibody |
| Recombinant DNA reagent |
pmAb-101F HC |
This work |
|
Plasmid for expressing recombinant antibody |
| Recombinant DNA reagent |
pmAb-101F LC |
This work |
|
Plasmid for expressing recombinant antibody |
| Recombinant DNA reagent |
pmAb-D25 HC Fab |
This work |
|
Plasmid for expressing recombinant antibody |
| Recombinant DNA reagent |
pmAb-D25 LC |
This work |
|
Plasmid for expressing recombinant antibody |
| Recombinant DNA reagent |
pmAb-3D3 HC |
This work |
|
Plasmid for expressing recombinant antibody |
| Recombinant DNA reagent |
pmAb-3D3 LC |
This work |
|
Plasmid for expressing recombinant antibody |
| Recombinant DNA reagent |
pmAb-J chain |
This work |
|
Plasmid for expressing recombinant antibody |
| Recombinant DNA reagent |
Lenti CRISPRv2 |
Addgene |
|
Ref. [70] |
| Biological sample (Homo sapiens) |
IgG/IgM-depleted normal human serum |
Pelfreeze |
34,014 |
|
| Biological sample (Homo sapiens) |
C3 |
Complement Technology, Inc |
A113 |
|
| Biological sample (Homo sapiens) |
C4 |
Complement Technology, Inc |
A105 |
|
| Biological sample (Homo sapiens) |
C1 |
Complement Technology, Inc |
A098 |
|
| Antibody |
Mouse anti-CD46 monoclonal (TRA-2–10) |
BioLegend |
BioLegend Cat# 352404, RRID:AB_10900243
|
IF (1:500) |
| Antibody |
Mouse anti-CD55 monoclonal (JS11) |
BioLegend |
BioLegend Cat# 311301, RRID:AB_314858
|
IF (1:500) |
| Antibody |
Mouse anti-C1q monoclonal (1A4) |
Santa Cruz Biotech. |
Santa Cruz Biotechnology Cat# sc-53544, RRID:AB_1119798
|
IF (1:500) |
| Peptide, recombinant protein |
Streptavidin |
Thermo Fisher Scientific |
434,302 |
|
| Peptide, recombinant protein |
CLPMTGG peptide |
Genscript |
|
Peptide for SrtA-based labeling |
| Peptide, recombinant protein |
Sortase A |
This work |
|
Recombinant SrtA for labeling |
| Peptide, recombinant protein |
Sfp synthase |
This work |
|
Recombinant Sfp for labeling |
| Chemical compound, drug |
Sulfo-Cy5 maleimide |
Lumiprobe |
Cat# 13,380 |
|
| Chemical compound, drug |
AF-488 NHS Ester |
Lumiprobe |
Cat# 11,820 |
|
| Chemical compound, drug |
Alexa Fluor 488 C5 maleimide |
Thermo Fisher Scientific |
Cat# A10254 |
|
| Chemical compound, drug |
Alexa Fluor 555 C2 maleimide |
Thermo Fisher Scientific |
Cat# A20346 |
|
| Chemical compound, drug |
NH2-PEG-Biotin |
Rapp Polymere |
Cat# 133000-25-20 |
|
| Chemical compound, drug |
CH3O-PEG-NH2 |
Rapp Polymere |
Cat# 122000–2 |
|
| Chemical compound, drug |
Coenzyme A trilithium salt |
Sigma-Aldrich |
Cat# C3019 |
|
| Software, algorithm |
Matlab R2020a |
Mathworks |
|
Used for data analysis, plotting, and simulations |
| Software, algorithm |
Nikon Elements |
Nikon |
|
Used for image acquisition |
| Software, algorithm |
PyMOL |
Schrödinger, Inc |
|
Used for structure alignment |
