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. 2021 Sep 16;8(6):rbab051. doi: 10.1093/rb/rbab051

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© The Author(s) 2021. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — The effect of pTa-GNPs hydrogel scaffold associated with BMSCs and BMSCs-derived ECs on angiogenesis. (a–d) Van Gieson’s staining of histological sections of four groups’ implants at 4 weeks post-operation revealed the presence of numerous blood vessels (a: control; b: BMSCs; c: endothelial differentiated BMSCs; d: endothelial differentiated BMSCs+BMSCs). White arrows marked the newly formed blood vessels. The large image was magnified ×100 with a scale bar = 100μm; the small image was magnified ×200 with a scale bar = 100μm (marked by white arrows). (e) Histomorphometric analysis of the samples after 28 days of implantation. Quantitative results of vascular lumens determined the microvessel density. Bars represent the mean microvessel density of four groups of implants ± SD. Data were analyzed by analysis of variance (ANOVA) followed by post-hoc multiple comparisons using Tukey's test. *P < 0.05.