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. 2021 Sep 20;11(18):e4167. doi: 10.21769/BioProtoc.4167

Figure 3. Example of Polysome Profiling.

Figure 3.

The absorbance of OD254 (A254) that represents the abundance of RNA fragments was measured by UA-6 detector. The sucrose gradient was fractionated into 17 fractions with Density gradient fractionation system. The value of A254 was recorded by Peak Chart and plotted as shown.