Table 1. Recommend schedule of this protocol.

Prepare 10× polysome buffer and 70% sucrose solution for long use.
Day 1	1	Seed cells.
Day 2	1	Prepare lysis buffer and wash buffer.
	2	Harvest samples following the steps of Sample preparation in order.
	3	Prepare sucrose gradient according to step A of the Procedure.
	4	Run polysome profiling following step C of the Procedure.
	5	Store the fractionated samples at -80°C.
Day 3	1	Thaw the samples on ice.
	2	Extract RNA and prepare RNA-seq library following Procedure D.
Sequencing
7-10 days	Run process for circRNAs identification