Figure 4.

Wogonin suppressed telomerase activity by inhibition of hTERT and c-Myc in wogonin-induced senescent cells. (a) Hut-102 and Jurkat cells were treated with or without 20 μM wogonin for 5 d. The relative mRNA level of hTERT and C-MYC was measured by RT-PCR. Columns represent the mean from three parallel experiments (mean ± SEM). ^∗p < 0.05, ^∗∗p < 0.01, compared with the control group. (b) Hut-102 and Jurkat cells were treated with or without 20 μM wogonin for 5 d. The expression of c-Myc protein was performed by western blot. β-Actin was used as loading controls. (c) Relative protein expression level of c-Myc. Columns represent the mean from three parallel experiments (mean ± SEM). ^∗p < 0.05, ^∗∗p < 0.01, compared with the control group. (d) Hut-102 and Jurkat cells were treated with or without 20 μM wogonin for 5 d. The expression of hTERT protein was performed by western blot. β-Actin was used as loading controls. (e) Relative protein expression level of hTERT. Columns represent the mean from three parallel experiments (mean ± SEM). ^∗p < 0.05, ^∗∗p < 0.01, compared with the control group.