Table 1.

Parameters for each immunoglobulin qualified in ELISA methodology.

Antibody of interest	Dilution of secondary antibody	Development time (minutes)	Initial plasma dilution per antibody
Total IgG⁎	1:4000	2.5–3.5	1:20
IgG1	1:1000	10	1:2
IgG3	1:1000	10	1:2
IgG4	1:2000	10	1:2
IgM	1:2000	3.5–4.5	1:20
IgA	1:8000	3.5–4.5	1:20

^⁎RBD qualification was only completed for total IgG.