Table 2.

ELISA intraday precision analysis using coefficient of variation (%).

Antigen	RBD	Spike
Sample ID	Total IgG	Total IgG	IgM	IgA	IgG1	IgG3	IgG4
A	7.21	2.40	5.04	1.95	8.86	7.47	ND
B	2.93	2.36	6.30	4.47	8.51	ND	ND
C	1.86	1.71	1.48	1.26	7.24	18.46	ND
D	1.84	2.28	2.02	3.38	6.80	16.75	ND
E	3.59	1.21	2.28	0.54	5.46	12.21	ND
F	0.86	0.82	5.19	1.67	4.67	13.3	2.68
G	2.14	1.41	1.68	2.27	7.97	9.92	ND
H	2.53	0.62	1.22	2.11	4.14	ND	ND
I	5.92	2.07	1.12	0.77	4.44	9.10	ND
J	2.44	1.04	1.00	1.30	4.92	13.24	ND
K	4.12	2.12	1.10	1.08	3.51	2.61	ND
L	0.004	1.65	2.20	1.57	6.86	9.72	ND
M	0.85	0.73	3.28	1.51	1.88	1.91	7.39
N	0.69	4.13	0.63	1.37	4.82	4.81	ND
CCPP	0.69	4.83	1.51	3.78	5.80	6.38	8.09
NHPP	0.28	0.05	7.09	0.12	3.07	0.00	10.14
Average	2.37	1.84	2.7	1.82	5.56	8.39	5.66
Standard Error	0.51	0.31	0.51	0.29	0.50	1.48	1.58

ND: not done, sample values below the limit of detection or not used for this assessment.