Table 4.
ELISA interoperator precision analysis using coefficient of variation (%).
| Antigen |
RBD |
Spike |
|||||
|---|---|---|---|---|---|---|---|
| Sample ID | Total IgG | Total IgG | IgM | IgA | IgG1 | IgG3 | IgG4 |
| A | 1.48 | 9.12 | 4.82 | 10.15 | ND | 3.66 | ND |
| B | 12.1 | 3.50 | 9.53 | 3.40 | ND | ND | ND |
| C | 5.03 | 7.15 | 10.82 | 12.49 | 10.53 | 6.05 | ND |
| D | 3.98 | 5.68 | 12.58 | 10.03 | 19.67 | ND | ND |
| E | 2.55 | 7.43 | 10.76 | 12.94 | 6.97 | 1.68 | ND |
| F | 2.08 | 0.42 | 9.28 | 8.39 | 8.54 | 4.65 | 7.02 |
| G | 4.05 | 2.38 | 12.59 | 12.34 | 8.72 | ND | ND |
| H | 1.33 | 15.18 | 7.00 | 5.76 | ND | 1.24 | ND |
| I | 1.46 | 2.94 | 9.63 | 13.41 | 14.67 | 7.85 | ND |
| J | 5.05 | 8.73 | 7.91 | 9.23 | ND | ND | ND |
| K | 3.06 | 7.91 | 11.85 | 14.50 | ND | 7.80 | ND |
| L | 3.77 | 5.61 | 7.42 | 7.33 | 19.33 | 3.35 | ND |
| M | 4.68 | 8.39 | 10.36 | 6.27 | 12.76 | 4.42 | 13.18 |
| N | 0.43 | 4.85 | 7.93 | 8.28 | 4.53 | 4.75 | ND |
| CCPP | 5.55 | 10.21 | 7.26 | 14.58 | 10.62 | 2.19 | 1.49 |
| NHPP | 0.92 | 0.19 | 1.26 | 0.84 | 1.29 | 0 | 13.2 |
| Average | 3.60 | 6.23 | 8.81 | 9.37 | 10.69 | 3.66 | 8.73 |
| Standard Error | 0.70 | 0.97 | 0.74 | 1.00 | 1.72 | 0.71 | 2.82 |
ND: not done, sample values below the limit of detection or not used for this assessment.