REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-GFP	Clontech	Cat# 632381; RRID: AB_2313808
Rabbit anti-Vinculin	Abcam	Cat# GR268234-50; RRID: AB_2241513
Mouse anti-FLAG M2	Sigma-Aldrich	Cat# SLBT654; RRID:AB_262044
Rabbit anti-RPS2	Bethyl labs	Cat# A303-794A-M; RRID:AB_2781471
Rabbit anit-RPS3	Proteintech	Cat# 11990-1-AP; RRID:AB_2180758
Rabbit anti-RPS24	Bethyl labs	Cat# A303-545A; RRID:AB_2620193
Rabbit anit-RACK1	Bethyl labs	Cat# A302-545A; RRID:AB_1999012
Rabbit anit-FLAG M2 (magnetic beads)	Sigma-Aldrich	Cat# M8823-5mL; RRID:AB_2637089
HRP goat anit-mouse IgG	SouthernBiotech	Cat# 1031-05; RRID:AB_2794307
HRP goat anti-rabbit IgG	SouthernBiotech	Cat# 4030-05; RRID:AB_2687483
Enzymes
EcoRI	New England Biolabs	R3101S (10,000U)
In-Fusion® snap assembly (master mix)	Clontech (TaKaRa)	638943
TURBO DNase	Invitrogen	AM2239 (5,000U)
AMV Reverse Transcriptase	Promega	M5101 (300U)
SuperScript IV RT	ThermoFisher	18090050 (10,000U)
PreScission protease	Cytiva	27084301
T7 RNA polymerase	New England Biolabs	M0251L (25,000U)
2′-O-Methyltransferase	New England Biolabs	M0366S (50,000U)
Vaccinia D1/D2 (Capping enzyme)	New England Biolabs	M2080S (400U)
Cell lines
BL21-CondonPlus (DE3)-RIPL	Agilent	230240
HEK293T cells	UC, Berkeley TC Core	N/A
HELA cells	UC, Berkeley TC Core	N/A
Chemicals, peptides, and recombinant proteins
Overnight ExpressTM instant TB medium	Novagen	71300-4
cOmpleteTM, EDTA-free protease inhibitor cocktail	Roche	5056489001
Halt Protease and Phosphatase inhibitor single-use	ThermoFisher	78444
Trichloroacetic acid (TCA)	Sigma-Aldrich	T0699-100ML
3XFLAG peptide	Sigma-Aldrich	F4799-4MG
TRIzol Reagent	ThermoFisher	15596026
GMP-PNP trisodium salt hydrate	Sigma-Aldrich	G0635-5MG
Cycloheximide (CHX)	Sigma-Aldrich	C4859-1ML
Glutathione Sepharose 4 (fast flow)	Cytiva	17513202
Kaposi’s sarcoma associated herpesvirus (ORF37)	This lab	PMID: 30321376
TAMRA maleimide, 6-isomer	Lumiprobe life science	28180 (5mg)
Murine RNase inhibitor	New England Biolabs	M0312L (15,000U)
Actinomycin D	Sigma-Aldrich	A1410-5MG
SUPERase⋅In RNase inhibitor	Invitrogen	AM2696 (10,00U)
Critical commercial assays
PolyJet (transfection reagent)	SignaGen® Labs	SL100688
Nano-Glo® Luciferase Assay kit	Promega	N1110
iTaq universal SYBR® green supermix	Bio-Rad	1725121
Nuclease-treated rabbit reticulocyte lysate (RRL)	Promega	L4960
Oligonucleotides
Primers for qPCRs, primer extension assay, and cloning	This paper	See Table S1
Recombinant DNA
pCDNA4TO-3XFLAG Halo WT nsp1	This paper	175412 (Addgene)
pCDNA4TO-3XFLAG Halo R124A/K125A nsp1	This paper	175417 (Addgene)
pCDNA4TO-3XFLAG Halo K164A/H165A nsp1	This paper	175421 (Addgene)
pCDNA4TO-3XFLAG Halo R99A nsp1	This paper	175422 (Addgene)
pCDNA4TO-3XFLAG Halo delta 1-117 nsp1	This paper	175423 (Addgene)
pCDNA4TO-3XFLAG Halo delta 118-180 nsp1	This paper	175424 (Addgene)
pCDNA4TO-3XFLAG Halo delta 122-130 nsp1	This paper	175425 (Addgene)
pCDNA4TO-3XFLAG Halo G-Linker nsp1	This paper	175426 (Addgene)
pCDNA4TO-3XFLAG Halo E55A E57A K58A nsp1	This paper	175427 (Addgene)
pCDNA4TO-3XFLAG Halo R119A K120A nsp1	This paper	175428 (Addgene)
pCDNA4TO-3XFLAG Halo E36A E37A nsp1	This paper	175429 (Addgene)
pJP-CoV2leader-nLuc-TSS	This paper	175430 (Addgene)
pJP-HBB-nLuc	This paper	175431 (Addgene)
pUC57-HBB-nLuc	This paper	175432 (Addgene)
pGEX nsp1 CoV2	This paper	175512 (Addgene)
pGEX nsp1 R124A/K125A CoV2	This paper	175513 (Addgene)
pGEX nsp1 K164A/H165A CoV2	This paper	175514 (Addgene)
pGEX nsp1 R99A CoV2	This paper	175515 (Addgene)
pGEX C_K nsp1 CoV2	This paper	175516 (Addgene)
pGEX C_K nsp1 K164A/H165A	This paper	175517 (Addgene)
pUC Ad VAI	This paper	175518 (Addgene)
pCDNA4 CoV-2 nsp1 3xFLAG	This paper	176057 (Addgene)
pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG	This paper	176059 (Addgene)
pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG	This paper	176060 (Addgene)
pCDNA4TO R99A CoV-2 nsp1 3xFLAG	This paper	176061 (Addgene)
Pd2EGFP-N1_plusSL	This lab	PMID: 22740404
Software and algorithms
GraphPad (Prism)	This paper	Version 9.2.0 (283)