TABLE 1.
Inter- and intra-assay variationsa
| No. of CMV particles/ml of plasma | Variation (mean no. of copies of CMV DNA/ml [% CV])
|
|
|---|---|---|
| Interassay | Intra-assay | |
| 150,000 | 151,908 (18) | 124,791 (36) |
| 50,000 | 51,346 (29) | 48,258 (39) |
| 16,665 | 18,213 (28) | 15,423 (27) |
| 5,556 | 6,720 (14) | 8,128 (8) |
| 1,851 | 3,231 (38) | 2,790 (24) |
| 617 | 940 (16) | 884 (19) |
| 206 | 336 (29) | 308 (22) |
a
Human reference plasma was supplemented with CMV to 150,000 copies/ml, and serial threefold dilutions were made in the same plasma. Q-PCR was done for these specimens to determine interassay (n = 7) and intra-assay (n = 8) variations.