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. 2021 Sep 29;12:5702. doi: 10.1038/s41467-021-25846-3

Fig. 6. CHD7-bound genes implicated in brain folding disorders are significantly conserved between perinatal mouse and fetal human cerebellum.

Fig. 6

a Immunohistochemistry analysis of 22 gestational week human cerebellum with antibodies recognizing hCHD7. Experiment was performed once on multiple sections. Scale bar: 100 µm. b Aggregate plot of ChIP-seq signal for hCHD7 (purple) and input (black) centered on hCHD7 genomic binding sites (n = 17,100). 22 gestational week anterior human cerebellum (n = 1 for CHD7 Ctrl, n = 1 for input). c Venn-diagram showing overlap of CHD7 mouse and human target genes with hypergeometric analysis. p-value =  4.48E-10, hypergeometric. See Methods section for more details on analysis. d Panther pathways analysis of mouse CHD7 direct target genes (left) and PMG-associated genes (right). Shared pathways are shown in red. e WashU Epigenome Browser view of two CHD7 direct target genes (TUBB2B, top; MAP1B, bottom) found in mouse and human cerebellum. Each site shows ChIP-seq coverage of CHD7 and H3K27ac. f H&E staining of 5-month-old clinically diagnosed CHARGE syndrome patient. Arrowheads point to sites or excessive ectopic cerebellar foliation. Zoom in of dashed rectangle shown below. EGL external granule layer; ML molecular layer; PCL Purkinje cell layer; IGL internal granule layer. Experiment was performed once on multiple sections for each region. Please refer to Methods for additional details regarding patient cerebellar sample.