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. 2021 Sep 29;12(10):888. doi: 10.1038/s41419-021-04193-7

Fig. 6. Hsa_circ_0043280 sponged miR-203a-3p to regulate the expression of PAQR3.

Fig. 6

A RIP assay showing the association between AGO2 and hsa_circ_0043280. Top, IP efficiency of the AGO2-antibody in Western blots. Bottom, relative enrichment representing RNA levels associated with AGO2 relative to the input control. An IgG antibody served as a control. B, C Schematic model and Sanger sequencing for wild-type or mutant transcripts of hsa_circ_0043280 or PAQR3 3′ UTR luciferase reporters (left). Luciferase reporter activity of hsa_circ_0043280 and the PAQR3 3′ UTR in HEK-293T cells co-transfected with miR-203a-3p mimics or mimics NC (right). D RAP assays showed that miR-203a-3p could be pulled down by a biotinylated probe designed for the hsa_circ_0043280 back-splicing site in MS751 and HeLa cells. E Hsa_circ_0043280 and PAQR3 mRNA were pulled down and enriched with the 3′-end biotinylated miR-203a-3p by miRNA capture assays. F RNA FISH indicated the colocalization of hsa_circ_0043280 and miR-203a-3p. Scale bar, 20 μm. G PAQR3 expression in MS751 and HeLa cells transfected with miR-203a-3p mimics alone or co-transfected with hsa_circ_0043280. Each experiment was performed at least three times independently. **P < 0.01; ***P < 0.001.