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. 2021 Sep 29;12(10):889. doi: 10.1038/s41419-021-04164-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A, B RT-qPCR assay detected the mRNA expression levels of 25 different kinases in Bel-7402 and Bel-7404 cells and detailed information on one overlapping gene. C CDK8 was analyzed by qPCR in Bel-7402 or Bel-7404 cells subjected to the indicated treatments. D, E CDK19 and CDK8 were analyzed by WB in Bel-7402 or Bel-7404 cells subjected to the indicated treatments. F Representative WB images of CDK19 in Bel-7402 and Bel-7404 cells (transfected with 1–40 μM CA). G, H Cell proliferation and colony-formation capacity were measured with CCK-8 cytotoxicity tests (G) and soft agar colony-formation assays (H), respectively, in Bel-7402 and Bel-7404 cells subjected to different treatments as indicated. Scale bar, 500 μm. Data are shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.