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. 2021 May 5;28(10):2888–2899. doi: 10.1038/s41418-021-00790-3

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© The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Immunoblotting analysis of RIP1, RIP3, and MLKL in WT and TRIM25 KO HT-29 cells. b FLAG-tagged RIP3 was transfected into HeLa cells, then followed by the treatment with siRNAs of TRIM25. c FLAG-tagged RIP3 was co-transfected with or without Myc-tagged TRIM25 into HeLa cells, followed by the treatment by adding 10 uM MG132. d FLAG-tagged RIP3 was co-transfected with Myc-tagged TRIM25 or its mutant TRIM25^CS into HeLa cells, followed by the treatment with adding 10 uM MG132. e TRIM25 or TRIM25^CS were co-transfected with RIP3 in a dose-dependent manner in HeLa cells. f FLAG-tagged RIP3 was co-transfected with or without Myc-tagged TRIM25 into HeLa cells, followed by the treatment with MG132 (10 uM) or chloroquine (10 uM) for 4 h.