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. 2021 May 5;28(10):2888–2899. doi: 10.1038/s41418-021-00790-3

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© The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a qRT-PCR analysis of TRIM25 or other E3 ligases (TRIM21 and CBL) treated with TNF/Z-VAD (TZ) for 4 h in L929 cells. Immunoblotting analysis of TRIM25 treated with TZ for indicated hours in L929 cells (b), and quantitative by using ImageJ (c). d Immunoblotting analysis of TRIM25 treated with TSZ for indicated hours in HT-29 cells. e Immunoblotting analysis of TRIM25 treated with TSZ for indicated hours in iBMDM cells. f Immunoblotting analysis of TRIM25 treated with TSZ or TNF in HT-29 cells. Immunoblotting analysis of TRIM25 treated with TZ or TNF/Z-VAD/Nec-1 (TZN) for 4 h in L929 cells (g), and quantitative by using ImageJ (h).