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. 2021 Sep 29;11:19283. doi: 10.1038/s41598-021-98831-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Bladder mucosal afferents are more mechano-sensitive during the day compared to the night. (A) Mucosal afferent response to mucosal stroking (10, 100, and 500 mg) at 0300 h and 1500 h. N = 6, n = 12 at each time point. ***P < 0.001, ****P < 0.0001. (B) An example of a raw trace of mucosal afferent responses to 100 mg stroking at 0300 h and 1500 h.