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. 2021 Sep 29;11:19283. doi: 10.1038/s41598-021-98831-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic of the ex vivo extracellular recording set up. A region of bladder (approximately 12 mm wide by 15 mm long, shown schematically by the dashed lines) cut off the full thickness bladder flat-sheet preparation together with attached nerves. The nerve trunks are carefully dissected of the connective tissue and placed in a paraffin oil bubble. The opposite side of the bladder preparation is attached to a hook and cantilever system for stretch by placing the imposed loads (1–40 g) which is also connected to an isotonic transducer for measuring bladder lengthening. A von Frey hair is stroked across receptive fields in the mucosa. The nerve trunks are individually placed onto a platinum electrode for electrical recordings.