FIG. 1.

SDS-polyacrylamide gel analysis of the ste18 mutant proteins. (A) C-terminal sequence of Ste18p precursor predicted from nucleic acid sequence. (B) Immunoblotting analysis performed on cleared lysates of exponentially growing cells. The STE18 allele designations are listed across the top. Amounts of protein loaded (from left) were as follows: ste18::URA3 (null), 33 μg; STE18⁺, 5 μg; ste18-C107S, 33 μg; STE18⁺, 5 μg; STE18⁺, 5 μg; ste18-C106S, 10 μg; and STE18⁺, 5 μg. Strains used were BRI1426, W303-1A, HC107S, and HC106S.