TABLE 1.
Strain list
| Straina | Genotype |
|---|---|
| W303-1A | MATa ade2 his3 leu2 trp1 ura3 can1 |
| HC106S | W303-1A ste18-C106S |
| HC107S | W303-1A ste18-C107S |
| BRI1426 | W303-1A ste18::URA3 |
| 809-A | W303-1A ram1::HIS3 |
| 810-A | W303-1A ram1::HIS3 ste18-C106S |
| 811-A | W303-1A ram1::HIS3 ste18-C107S |
| 814-A | W303-1A ram1::HIS3 ste18::URA3 |
| 816-1/M70p2 | W303-1A ste18::ura3 containing plasmid M70p2 |
| 816-1/M70p2C106S | W303-1A ste18::ura3 containing plasmid M70p2C106S |
| 816-1/M70p2C107S | W303-1A ste18::ura3 containing plasmid M70p2C107S |
| 817-5/M70p2 | W303-1A ste18::ura3 ram1::HIS3 containing plasmid M70p2 |
| 817-5/M70p2C106S | W303-1A ste18::ura3 ram1::HIS3 containing plasmid M70p2C106S |
| 817-5/M70p2C107S | W303-1A ste18::ura3 ram1::HIS3 containing plasmid M70p2C107S |
| W303 (diploid) | MATa/MATα ade2/ade2 his3/his3 leu2-/leu2 trp1/trp1 ura3/ura3 can1/can1 |
a
Strains HC106S and HC107S were from M. S. Whiteway (48). Strain BRI146 was also kindly provided by M. S. Whiteway. All other strains were generated during the course of this study.