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. 2021 May 4;28(10):2871–2887. doi: 10.1038/s41418-021-00789-w

Fig. 1. SNX3 expression was increased in failing human hearts, and cardiac-specific Snx3-cKO mice were protected from ISO-induced cardiac injury.

Fig. 1

A and B A significant induction of SNX3 protein expression in end-stage failing human heart tissues (n = 9 per HF, n = 3 per normal control), which was identified by IF assay (Scale bar: 200 μm) and western blot analysis. Male C57BL/6 Snx3-cKO mice and their respective CTL at 11 weeks were injected with ISO (3 mg/kg/day, s.c.) or an equal volume of sterile normal saline for one week. C The protein expression of SNX3 was measured by an IF assay (Scale bar: 100 μm). D and E Gross observation of heart morphology, WGA staining (Scale bar: 50 μm)-stained cross-sections of heart tissues were shown. F The HW/TL ratio was calculated. G Masson staining (Scale bar: 100 μm)-stained cross-sections of heart tissues were shown. H The representative echocardiographic graphs were presented. I The echocardiographic parameters IVS was measured. Representative images of five independent experiments were presented. The data were presented as the means ± SEM. *P < 0.05 vs. Normal or CTL + Saline group, #P < 0.05 vs.. CTL + ISO group, n = 11 per CTL + Saline group, n = 11 per cKO + Saline group, n = 10 per CTL + ISO group, n = 12 per cKO + ISO group. CTL littermate negative control, HF heart failure, HW/TL, the heart weight to the tibia length ratio, IF immunofluorescence, ISO isoproterenol, IVS interventricular septum, ns no statistical difference, s.c. subcutaneously, Snx3-cKO cardiac-specific Snx3 knockout, WGA wheat germ agglutinin, 1w 1 week. See also Supplementary Figs. S1–S4.