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. 2021 May 4;28(10):2871–2887. doi: 10.1038/s41418-021-00789-w

Fig. 5. SNX3–retromer promoted the nuclear localization of STAT3 in NRCMs.

Fig. 5

NRCMs were infected with Ad-sh-SNX3 or Ad-SNX3 before ISO treatment for 1 h. Besides, another group of NRCMs was infected with Ad-SNX3 followed by transfection with shRNAs of importin α3. A, E, and F IF assay was performed to detect the subcellular distribution of STAT3, SNX3, VPS35, and VPS26. Representative images of five independent experiments were presented. B and G The nuclear and cytoplasmic proteins were extracted from NRCMs, and were detected by western blot analysis. The results were normalized to those of α-tubulin/Lamin B1. C Luciferase reporter gene assays showed the transcriptional activity of STAT3. D The mRNA levels of the target genes of STAT3 (c-myc, bcl-xl, and aGT) were confirmed by qPCR. The data were shown as the means ± SEM. *P < 0.05 vs. NC or Ad-Flag group, #P < 0.05 vs.. Ad-sh-SNX3 or Ad-SNX3 group, n = 5. IF immunofluorescence, ISO isoproterenol, NC negative control, NRCMs neonatal rat cardiomyocytes, qPCR quantitative polymerase chain reaction. See also Supplementary Figs. S11–S16.