Table 1.
Predicted ORFs, their positions on the DRL-P1 genome, size, annotations and probable role in phage life cycle.
| REGIONs/ORFs | Span | Strand | Length | GenBankannotation | Role/function in phage life cycle | ||
|---|---|---|---|---|---|---|---|
| Start | Stop | ORF | aa | ||||
| REGION 1 | 1 | 353 | + | 353 | Terminal repeat | Genome replication & packaging | |
| ORF1 | 634 | 3216 | − | 2583 | 860 | Phage internal (Core) protein | Virion Structure |
| ORF2 | 3220 | 3648 | − | 429 | 142 | Phage exonuclease | Genome replication |
| ORF3 | 3658 | 4251 | − | 594 | 197 | Phage tail fiber protein | Host infection |
| ORF4 | 4260 | 4799 | − | 540 | 179 | Hypothetical protein | |
| ORF5 | 4799 | 5302 | − | 504 | 167 | Phage tail fiber protein | Host infection |
| ORF6 | 5312 | 5740 | − | 429 | 142 | Hypothetical protein | |
| ORF7 | 5742 | 6092 | − | 351 | 116 | Phage exonuclease | Replication |
| ORF8 | 6089 | 6412 | − | 324 | 107 | Hypothetical protein | |
| ORF9 | 6412 | 6864 | − | 453 | 150 | Phage tail fiber Protein | Host infection |
| ORF10 | 6922 | 8436 | − | 1515 | 504 | Putative transcriptional regulator | Gene expression |
| ORF11 | 8453 | 9034 | − | 582 | 193 | Hypothetical protein | |
| ORF12 | 9031 | 9582 | − | 552 | 183 | Hypothetical protein | |
| ORF13 | 9590 | 9988 | − | 399 | 132 | Hypothetical protein | |
| ORF14 | 9985 | 10,452 | − | 468 | 155 | Hypothetical protein | |
| ORF15 | 10,467 | 10,904 | − | 438 | 145 | Hypothetical protein | |
| ORF16 | 11,006 | 12,154 | − | 1149 | 382 | Phage capsid and scaffold Protein | Virion assembly |
| ORF17 | 12,164 | 12,799 | − | 636 | 211 | Hypothetical protein | |
| ORF18 | 12,803 | 14,230 | − | 1428 | 475 | Phage capsid and Scaffold protein | Virion assembly |
| ORF19 | 14,743 | 14,883 | − | 141 | 46 | Hypothetical protein | |
| ORF20 | 14,880 | 15,086 | − | 207 | 68 | Hypothetical protein | |
| ORF21 | 15,106 | 15,942 | − | 837 | 278 | Phage minor capsid protein | Virion Structure & assembly |
| ORF22 | 15,942 | 18,239 | − | 2298 | 765 | putative minor head protein | Virion Structure |
| ORF23 | 18,419 | 18,820 | + | 402 | 133 | Hypothetical protein | |
| ORF24 | 18,852 | 19,175 | + | 324 | 107 | Hypothetical protein | |
| ORF25 | 19,172 | 19,375 | + | 204 | 67 | Hypothetical protein | |
| ORF26 | 19,381 | 19,692 | + | 312 | 103 | Hypothetical protein | |
| ORF27 | 19,776 | 20,288 | + | 513 | 170 | Hypothetical protein | |
| ORF28 | 20,392 | 21,324 | + | 933 | 310 | Hypothetical protein | |
| ORF29 | 21,321 | 21,416 | + | 96 | 31 | Hypothetical protein | |
| ORF30 | 21,426 | 22,019 | + | 594 | 197 | Hypothetical protein | |
| ORF31 | 22,036 | 22,473 | + | 438 | 145 | Hypothetical protein | |
| ORF32 | 22,559 | 23,338 | + | 780 | 259 | Hypothetical protein | |
| ORF33 | 23,341 | 23,775 | + | 435 | 144 | Hypothetical protein | |
| ORF34 | 23,820 | 24,170 | + | 351 | 116 | Hypothetical protein | |
| ORF35 | 24,170 | 24,388 | + | 219 | 72 | Hypothetical protein | |
| ORF36 | 24,385 | 24,768 | + | 384 | 127 | Hypothetical protein | |
| ORF37 | 24,805 | 26,187 | − | 1383 | 460 | Phage terminase, large subunit | DNA translocation and packaging termination |
| ORF38 | 26,387 | 26,575 | + | 189 | 62 | Hypothetical protein | |
| ORF39 | 26,695 | 26,841 | + | 147 | 48 | Hypothetical protein | |
| ORF40 | 26,852 | 27,154 | + | 303 | 100 | Hypothetical protein | |
| ORF41 | 27,201 | 28,118 | + | 918 | 305 | Phage tail length tape-measure protein | Genome injection |
| ORF42 | 28,121 | 28,309 | + | 189 | 62 | Hypothetical protein | |
| ORF43 | 28,389 | 28,574 | + | 186 | 61 | Hypothetical protein | |
| ORF44 | 28,699 | 28,899 | + | 201 | 66 | Hypothetical protein | |
| ORF45 | 28,896 | 29,111 | + | 216 | 71 | Hypothetical protein | |
| ORF46 | 29,108 | 29,299 | + | 192 | 63 | Hypothetical protein | |
| ORF47 | 29,296 | 29,508 | + | 213 | 70 | Hypothetical protein | |
| ORF48 | 29,536 | 30,183 | + | 648 | 215 | Hypothetical protein | |
| ORF49 | 30,180 | 30,506 | + | 327 | 108 | Putative single-stranded DNA binding protein | Genome replication |
| ORF50 | 30,572 | 30,796 | + | 225 | 74 | Hypothetical protein | |
| ORF51 | 30,850 | 31,077 | + | 228 | 75 | Phage dihydrofolate reductase | DNA synthesis |
| ORF52 | 31,087 | 31,308 | + | 222 | 73 | Hypothetical protein | |
| ORF53 | 31,356 | 31,673 | + | 318 | 105 | Phage single-stranded-DNA-specific exonuclease | Genome replication |
| ORF54 | 31,683 | 32,309 | + | 627 | 208 | Phage putative head protein | Virion Structure |
| ORF55 | 32,502 | 33,116 | + | 615 | 204 | Hypothetical protein | |
| ORF56 | 33,279 | 33,857 | − | 579 | 192 | Hypothetical protein | |
| ORF57 | 34,388 | 34,618 | − | 231 | 76 | Hypothetical protein | |
| ORF58 | 34,817 | 36,547 | − | 1731 | 576 | Phage-associated DNA primase | Genome replication |
| ORF59 | 36,695 | 36,880 | − | 186 | 61 | Hypothetical protein | |
| ORF60 | 36,886 | 37,962 | − | 1077 | 358 | Hypothetical protein | |
| ORF61 | 37,959 | 38,408 | − | 450 | 149 | Hypothetical protein | |
| ORF62 | 38,408 | 39,250 | − | 843 | 280 | Hypothetical protein | |
| ORF63 | 39,381 | 40,166 | − | 786 | 261 | Hypothetical protein | |
| ORF64 | 40,334 | 40,756 | + | 423 | 140 | Hypothetical protein | |
| ORF65 | 40,743 | 41,930 | + | 1188 | 395 | Capsid decoration protein | Virion Structure |
| ORF66 | 42,092 | 42,982 | + | 891 | 296 | Hypothetical protein | |
| ORF67 | 43,087 | 44,088 | + | 1002 | 333 | Hypothetical protein | |
| ORF68 | 44,178 | 44,408 | + | 231 | 76 | Hypothetical protein | |
| ORF69 | 44,408 | 44,626 | + | 219 | 72 | Hypothetical protein | |
| ORF70 | 44,610 | 44,828 | + | 219 | 72 | Phage tail assembly protein | Virion Structure |
| ORF71 | 44,828 | 45,094 | + | 267 | 88 | Hypothetical protein | |
| ORF72 | 45,106 | 45,312 | + | 207 | 68 | Phage minor tail protein | Virion Structure |
| ORF73 | 45,312 | 46,229 | + | 918 | 305 | Thymidylate synthase | DNA synthesis |
| ORF74 | 46,231 | 46,422 | + | 192 | 63 | Phage tail assembly protein | Virion Structure |
| ORF75 | 46,425 | 47,465 | + | 1041 | 346 | 5'Polynucleotide kinase-3'phosphatase | DNA damage repair |
| ORF76 | 47,541 | 48,095 | + | 555 | 184 | Hypothetical protein | |
| ORF77 | 48,095 | 51,202 | + | 3108 | 1035 | Phage DNA polymerase III alpha subunit | Genome replication |
| ORF78 | 51,195 | 51,605 | + | 411 | 136 | Phage Recombination protein | General recombination |
| ORF79 | 51,602 | 53,161 | + | 1560 | 519 | Phage DNA Helicase | Genome replication |
| ORF80 | 53,256 | 53,876 | + | 621 | 206 | Phage tail fiber protein | Virion Structure / Host infection |
| ORF81 | 53,965 | 54,861 | + | 897 | 298 | Hypothetical protein | |
| ORF82 | 54,917 | 55,522 | + | 606 | 201 | Hypothetical protein | |
| ORF83 | 55,519 | 56,073 | + | 555 | 184 | Phage DNA Binding protein | Genome replication |
| ORF84 | 56,127 | 57,038 | + | 912 | 303 | Phage DNA Ligase | Genome replication |
| ORF85 | 57,318 | 57,569 | + | 252 | 83 | Hypothetical protein | |
| ORF86 | 57,594 | 58,256 | − | 663 | 220 | Phage endolysin | Host cell Lysis |
| ORF87 | 58,256 | 58,684 | − | 429 | 142 | Phage tail fiber component | Virion Structure |
| ORF88 | 58,687 | 61,581 | − | 2895 | 964 | Phage tail fiber protein | Virion Structure |
| ORF89 | 61,586 | 63,100 | − | 1515 | 504 | Hypothetical protein | |
| ORF90 | 63,097 | 64,350 | − | 1254 | 417 | Phage tail assembly protein | Virion Structure |
| ORF91 | 64,407 | 65,072 | − | 666 | 221 | Baseplate protein | Virion Structure |
| ORF92 | 65,128 | 65,661 | − | 534 | 177 | Hypothetical protein | |
| ORF93 | 65,661 | 66,128 | − | 468 | 155 | Phage minor tail protein | Virion Structure |
| REGION2 | 65,891 | 66,243 | + | 353 | Terminal Repeat | Genome replication & packaging | |