Figure 2.

mRNA expression of ECMs in 2D cultured HTM cells. Among the experimental groups treated with or without 250 nM DEX or 5 ng/ml TGFβ2, 2D cultured HTM cells at Day 6 were subjected to a qPCR analysis to estimate the expression of mRNA of ECMs including COL 1, COL 4, COL 6, FN and a-SMA. All experiments were performed in duplicate using fresh preparations (n = 4). Data are presented as the arithmetic mean ± standard error of the mean (SEM). *P < 0.05; **P < 0.01; ***P < 0.005 (ANOVA followed by a Tukey’s multiple comparison test).