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Immunolabeling of ECMs of the 2D cultured HTM cells. Among the experimental groups treated with or without 250 nM DEX or 5 ng/ml TGFβ2, 2D HTM cells at Day 6 were subjected to an immunostaining of COL 1, COL 4, COL 6, FN and a-SMA. All experiments were performed in duplicate using fresh preparations (n = 4). Demonstrative images are shown (ECM: green, DAPI: blue).
