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. 2021 Sep 29;11:19369. doi: 10.1038/s41598-021-98766-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Zymography of HTM 2D cells and 3D spheroids. Among the experimental groups treated with or without 250 nM DEX or 5 ng/ml TGFβ2, HTM 2D cells and 3D spheroids at Day 6 were subjected to zymography measurements (panel A). The corresponding MMP band areas were evaluated using Image J soft (panel B). All experiments were performed in duplicate using fresh preparations (n = 3). Data are presented as the arithmetic mean ± standard error of the mean (SEM). *P < 0.05; **P < 0.01; ***P < 0.005 (ANOVA followed by a Tukey’s multiple comparison test).