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. 2021 Sep 29;11:19325. doi: 10.1038/s41598-021-98887-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Measurements of serum electrolytes and enzymes of animals exposed to 2 h (circles, solid line) or 3 h (triangles, dashed lines) of ischemia and different periods of reperfusion. Na + (A), K + (B), Cl− (C), urea (D), creatinine (E), ALP (F), AST (G), ALT (H), CK (I) and LDH (J). Units are milliequivalents per litre (mEq/L) for Na⁺, K⁺ and Cl⁻, mg/dL for urea and creatinine, and international units per litre (U/L) for ALP, AST, ALT, CK, and LDH. The asterisks indicate statistically significant differences when comparing the two durations of ischemia (2 or 3 h) in each reperfusion period analysed; *(p < 0.05), **(p < 0.01). ALP alkaline phosphatase, AST aspartate aminotransferase, ALT alanine aminotransferase, CK creatine kinase, LDH lactate dehydrogenase.